Steps in Recombinant DNA technology or rDNA technology
Jan 14, 2023
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00:00 | Introduction
00:10 | Definition of Recombinant DNA technology or rDNA technology
00:43 | Summary of steps in rDNA technology
01:25 | Step 1 identification and isolation of gene of interest
From where we get our gene of interest?
02:15 | Step 2: Insertion of this isolated gene in a suitable vector using restriction enzyme and ligase.
02:26 | What is a gene cloning vector? what is called rDNA molecule?
04:10 | Step 3: Introduction of this vector into a suitable organism/ cell called host (transformation)
Gene transfer methods- Physical and chemical gene transfer methods
05:23 | Step 4: Selection of the transformed host cell.
06:35| Step 5: Multiplication or expression of the introduced gene in the host.
Watch Steps Involved in the production of Recombinant Insulin. How Recombinant Insulin is produced? https://youtu.be/rq18lAEjtgI
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Detailed explanation of steps in recombinant DNA technology explained with the example of insulin synthesis in bacterium.
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