5 Steps in CRISPER/Cas 9 Gene Editing Technology || 3 Minute
Jul 16, 2023
3 minute video on 5 Steps in CRISPER/Cas 9 Gene Editing
Detailed video on Crisper Cas 9: https://youtu.be/iUULfg7FkiY
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00:00|| Introduction What is CRISPR/Cas9 gene editing?
00:10|| How is Crisper cas 9 used to treat Sickle cell disease?
00:49|| Step 1: HSPCs isolated and cultured
01:08|| Step 2: Synthesis of guide RNA
01:34|| Step 3: Transfection of cas 9 gRNA complex
02:16|| Step 4: DNA repair and editing
02:41|| Step 5: Infusion of edited HSPCs
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