1. Differences in absorption of left and right-handed circularly polarized light is measured by:
a) NMR spectroscopy
b) Mass spectroscopy
c) CD (Circular Dichroism) spectroscopy
d) UV vis spectrscopy
2. Full form of MALDI -MS is
a) Mass-assisted local degradation/ionic mass spectrometry
b) Mass-assisted local desoription/ionise mass specrometry
c) Matrix- assisted light deviation/ionization mass spectrometry
d)Matrix -assisted laser desorption/ionization mass spectrometry
3) The radiation source in TEM (Transmission Electron Microscopy) is
a) UV-light
b)Microwaves
c) Electron beam
d) Visible light
4) Which of the following is not a fluorescence dye?
a)Alexa fluor
b)Green Florescent Protein (GFP)
c)Xylene cyanole
d)Red Florescent Protein (DsRed)
5) The microscope that converts slight difference in refrective index and cell density into easily observed difference in the light intensity is known as-
a)Phase Contrast microscope
b)Bright field microscope
c)Dark field microscope
d)Transmission electron microscope
6. Vertical gel electrophoresis is routinely used for the separation of
a) Protein
b)DNA
c)RNA
d)Carbohydrate and Lipid
7. Two dimensional electrophoresis resolve the proteins on the basis of
a)Net mass and osmotic pressure
b)Net Viscosity and Volume
c) Net charge and Mass
d) Net charge and Volume
8.The pH at which net charge of protein becomes zero under an electrical field at room temperature is known as
a)isometric point
b)isothermal point
c)isoelectric point
d)isoelectric potential
9. The most common initial step to separate water-soluble proteins from insoluble cellular material is
a) electroporation
b) western blotting
c) differntial centrifugation
d) density gradient centrigugation
10. Which of the following techniques may be used for identifying a regularly protein that acts by binding a specific sequence of DNA?
a) Southern blotting
b) Northern blotting
c) Western blotting
d) South Western blotting
Answers:
1.c) CD (Circular Dichroism) spectroscopy
2. d)Matrix -assisted laser desorption/ionization mass spectrometry
3.c) Electron beam
4. c)Xylene cyanole
5. a)Phase Contrast microscope