1. In spectrophotometric measurement of proteins, which of the following amino acids contribute the maximum
a. Tyrosine, Phenylalanine, Tryptophan
b.Tyrosine, Serine, Threonine
c.Phenylalanine, Alanine, Leucine
d.Histidine, proline, Tryptophan
2. For respiration of two proteins with the same molecular weight, which of the following techniques could be used:
A. SDS-PAGE
B.2D-SDS-PAGE
C. Mass spectometry with liquid chromatography
D.Gel Flitration
E. Gel -filtration and SDS-PAGE
Choose the correct answer from the options given below:
a) E, A only
b) A, D only
c)A, B only
d) B and C only
3. Serum albumins and globulins can be separated by the method of:
a. Dialysis
b.Polarography
c.Spectrography
d.Electrophoresis
4. Which of the following molecules binds strongly to avidin?
a. Pantothenic acid
b.Biotin
c.Folic acid
d.Niacin
5. One of the following is not related to enzyme-linked immunosorbent assay (ELISA)
a. Monoclonal and polyclonal antibodies
b.Antigen and Coupling enzyme
c. Electrophoresis
d.Secondary antibody
6. Restriction enzymes are the enzyme that:
A. catalyse restricted reactions in DNA replication
B. are essential tools in biotechnology
C.were were discovered by Sanger and Pauling
D.cut DNA specifically
E. are the immune defense mechanism in bacteria
choose the correct answer from the options given below:
a. B, D, E only
b. C, D, E only
c. A,B only
d. C, D only
7. If you have to find out whether a given protein is bound to a given gene in chromatin, which technique would you prefer using:
a. Immunoprecipitation
b.DNasefootprinting
c.ChIP
d.SELEX
8. In analogy with central dogma of biology, how will you arrange the following omics in a correct sequence:
A.Metabolomics
B.Genomics
C.Proteomics
D.Transcriptomics
E. Phenotype or Phenomics
Choose the correct answer from the options given below:
a) B,C,D,E,A
b) C,B,D,A,E
c)C,D,B,A,E
d)C, E,B, A, D
9. Match the following
List 1 List 2
A. X-ray crystallography I. Molecular weight determination
B. Mass spectrometry II. Protein detection
C. Gel fitration III. Protein structure
D. Western blotting IV. Protein Purification
Choose the correct answer from the options given below:
a)A-II, B-I, C-III, D-IV
b)A-IV, B-III, C-II, D-I
c)A-I,B-II, C-III, D-IV
d)A-III, B-I,C-IV,D-II
10. Match the following
List 1 List 2
A. Hydrolase I. Acetyl CoA synthetase
B. Oxidoreductase II. Pyruvate kinase
C. Transferase III. Cytochtome oxidase
D. Ligase IV. Lipase
Choose the correct answer from the options given below:
a)A-I, B-IV, C-III, D-II
b)A-IV, B-II, C-II, D-I
c)A-I,B-II, C-III, D-IV
d)A-IV, B-III,C-I,D-II
Answers:
1. a. Tyrosine, Phenylalanine, Tryptophan
2. c)A, B only
3. d.Electrophoresis
4. b.Biotin
5. c. Electrophoresis
6. b. C, D, E only
7.c.ChIP
8.c)C,D,B,A,E
9.d)A-III, B-I,C-IV,D-II
10.d)A-IV, B-III,C-I,D-II