Microscopic Techniques Practice Test | Methods in Biology

1.Minimum resolution of electron microscope is
0.2nm
20nm
0.2mm
100nm

2.In electron microscopy, to dect specific macromolecule or structure such as spindle pole body (SPB),the frequently used procedure is to couple secondary antibody with
Alexa 568
Cy5
Gold Particle
Osmium tetraoxide

3. Ferritin Tagged antibody can be visualised through...
Autoradiography
Electron Microscopy
Phase Contrast Microscopy
Fluorescence Microscopy

4.Which of the following components is typicaly Not utilized while capturing image using Confocal Laser Scanning Microscope(CLSM)
CCD camera
Pinhole
Laser
Detectors
5.Fluorescence microscope that requires photoactivatable probes to obtain super resolution is
structured illumintion microsscope(SIM)
dSTORM-stochastic optical reconstruction microscopy
Laser scanning confocal microscope
stimulated emission depletion microscopy(STED)

6.The microscopic technique used for visualisation of 3D fluorescent structure of live biological sample is
Scanning electron microscopy
Transmission electron mcicroscopy
Laser confocal microscopy
Phase contrast microscope

7. Which of the following sets of terms is matched correcly?
Flouorescence microscopy: uses of dyes that absorb and emit light of same wavelength.
confocal microscopy: uses of pinpoint illumination and dectection apertures
Gas chromatography: used for analysis of non volatlised carbohydrate molcecules
None of these

8.Which one of the following microscopes would you use to visualise a protein fused to an appropriate reporter in a living cell?
fluorescence microscope
Scanning electron microscope
differential interference contrast microscope
phase contrast microscope

9.Colocalization of two fluoresently labelle dproteins in an organelle in cells is usually visualised by
interference contrast microscope
Scanning electron microscope
atomic force microscopy
confocal microscopy

10. Electron microscopes have much higher resolution than any type of light microscope because
of their higher magnification
the lenses used are of much higher quality
of very short wave length of electrons
the images are viewed on screen rather than direcly using an eye piece or occular lens

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