Spectroscopy is a technique used for the study of interaction of electromagnetic radiation with matter. It is used for the identification of substances through the spectrum emitted from or absorbed by them. It is used for the estimation of concentration of coloured compounds, analysis of chemical structure of molecules, types of molecular changes occurring during various enzymatic reactions; and in the study of intermolecular bonding. List of various spectroscopic techniques is given in Table
Spectrophotometric techniques offer a very rapid and convenient means of qualitative and quantitative estimation of biomolecules. Spectrophotometer consists of the following parts: light source, which provide the desired wavelength of light, collimator, which transmits a straight beam of light, monochromator, which splits the light into its component wavelength, and wavelength selector, that transmits only the desired wavelength.
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