Cloning Plasmids - Used to facilitate the cloning of DNA fragments. Cloning vectors tend to be very simple, often containing only a bacterial resistance gene, origin and Multiple cloning sites.
Expression Plasmids - Used for gene expression. Expression vectors must contain a promoter sequence as well as a transcription terminator sequence, and the inserted gene. An expression vector can also include an enhancer sequence which increases the amount of protein or RNA produced.
Gene knock-down Plasmids - Used for reducing the expression of an endogenous gene. This is frequently accomplished through expression of an shRNA (small hairpin RNA) targeting the mRNA of the gene of interest.
Reporter Plasmids - Used for studying the function of genetic elements. These plasmids contain a reporter gene (for example, luciferase or GFP) that offers a read-out of the activity of the genetic element. For instance, a promoter of interest could be inserted upstream of the luciferase gene to determine the level of transcription driven by that promoter.
Viral Plasmids - These plasmids use technology evolved by viruses to aid in delivery of genetic material into target cells . You can use these plasmids to create viral particles, such as lentiviral, retroviral, AAV, or adenoviral particles, that can infect target cells at a high efficiency.
Expression Plasmids - Used for gene expression. Expression vectors must contain a promoter sequence as well as a transcription terminator sequence, and the inserted gene. An expression vector can also include an enhancer sequence which increases the amount of protein or RNA produced.
Gene knock-down Plasmids - Used for reducing the expression of an endogenous gene. This is frequently accomplished through expression of an shRNA (small hairpin RNA) targeting the mRNA of the gene of interest.
Reporter Plasmids - Used for studying the function of genetic elements. These plasmids contain a reporter gene (for example, luciferase or GFP) that offers a read-out of the activity of the genetic element. For instance, a promoter of interest could be inserted upstream of the luciferase gene to determine the level of transcription driven by that promoter.
Viral Plasmids - These plasmids use technology evolved by viruses to aid in delivery of genetic material into target cells . You can use these plasmids to create viral particles, such as lentiviral, retroviral, AAV, or adenoviral particles, that can infect target cells at a high efficiency.