Serial Dilution Protocol PDF

It is a method of diluting a stock solution where concentration decreases by the same quantity in each successive step.
Materials required: stock solution, test tubes, pipettes, beaker, and distilled water.
Procedure:
Take 4 test tubes label A, B, C and D
Pour 9 ml of distilled water to these test tubes
Transfer 1ml of stock solution to the test-tube labeled A and mix well
Transfer 1 ml of solution from test tube A to test tube B and mix well
Transfer 1 ml of solution from test tube B to test tube C and mix well
Transfer 1 ml of solution from test tube C to test tube D and mix well and continue up to the required dilution. Here we are making 10^-4 dilution.

Serial Dilution Procedure PDF Click here

Inference:
Test tube A has 10 times dilution or 1/10^th or 10^-1 dilution of the stock solution.
Test tube B has 100 times dilution or 1/100^th or 10^-2 dilution
Test tube A has 1000 times dilution or 1/1000^th or 10^-3 dilution
Test tube A has 10000 times dilution or 1/10000^th or 10^-4 dilution 

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