DBT BET JRF Previous Solved Questions 2012

61. Holiday junction is observed during

(A) Mitosis
(B) Interphase
(C) Recombination
(D) DNA Repair

62. Okazaki fragments are

(A) RNA primers for DNA synthesis 7
(B) Short DNA fragments after nuclease digestion
(C) Newly synthesized DNA fragments
(D) Short stretches of DNA attached to RNA primers on lagging strand

63. Which one of the following antibiotics attaches to 50S ribosome and inhibits its peptidyl-transferase activity?

(A) Penicillin
(B) Chloramphenicol
(C) Trimethoprim
(D) Amphotericin

64. DNA from a host sample can be amplified by a process known as the polymerase chain reaction (PCR). Which of the following is required for PCR?

(A) Knowledge of the genetic sequence to be amplified
(B) A single nucleotide primer
(C) A universal probe to detect the amplified product

(D) A heat-sensitive DNA polymerase enzyme

65. Glycosylation of proteins occurs in the

(A) peroxisome
(B) Mitochondrion
(C) lysosome
(D) endoplasmic reticulum

66. The 20 different amino acids found in proteins are normally coded by

(A) 59 codons
(B) 60 codons
(C) 61 codons
(D) 63 codons

67. Which of the following is NOT found inside the eukaryotic nucleus?

(A) Nucleolus
(B) Cajal bodies
(C) PML bodies
(D) Centrosomes

68. A recombinant vaccine is available for which one of the following cancers?

(A) Adult T cell leukemia
(B) Colon carcinoma
(C) Glioblastoma
(D) Cervical carcinoma

69. The wavelengths of light that penetrate the least into the ocean are

(A) red and violet
(B) red and yellow
(C) blue and brown
(D) green and blue

70. WebIn is a sequence submission tool provided by

(A) NCBI 4
(B) EMBL
(C) EBI
(D) RCSB

71. National Center for Biotechnology Information (NCBI) was established on November 4, 1988 as a division of the

(A) National Library of Medicine (NLM)
(B) National Institutes of Health (NIH)
(C) European Bioinformatics Institute
(D) ExPASy

72. Which one of the following promoters is not derived from Agrobacterium?

(A) CaMV 35S
(B) nos
(C) ipt
(D) virD

73. In cell cycle, paternal and maternal chromosomes exhibit a “bouquet stage” during

(A) Leptotene
(B) Zygotene
(C) Pachytene
(D) Diplotene

74. Which of the following enzymes is NOT used in pyrosequencing?

(A) DNA Polymerase
(B) Pyrophosphatase
(C) Luciferase
(D) ATP sulfurylase

75. HSP70 chaperons are not present in which among the following organelles?

(A) Endoplasmic reticulum
(B) Golgi bodies
(C) Nucleus
(D) Mitochondria 

Learn more:

• Previous Solved DBT  Questions: 1- 20/ 21-40/ 41-60
• GATE-Biotechnology Questions : 1-15 /15-40
• Biotechnology MCQs and more

Answers:
61. (C) Recombination Refer: Holliday Model
62. (D) Short stretches of DNA attached to RNA primers on lagging strand
63. (B) Chloramphenicol  Refer: Protein synthesis inhibits
64. (D) A heat-sensitive DNA polymerase enzyme
65. (D) endoplasmic reticulum
66. (C) 61 codons (61 codons + 3 stop codons=64 codons)
67. (D) Centrosomes
68. (D) Cervical carcinoma
69. (D) green and blue
70. (B) EMBL
71. (A) National Library of Medicine (NLM) The National Center for Biotechnology Information (NCBI) is part of the United States National Library of Medicine (NLM), a branch of the National Institutes of Health.
72. (A) CaMV 35S
73. (B) Zygotene
74. (B) Pyrophosphatase
75. (C) Nucleus