ICAR ARS NET Questions on Agricultural Biotechnology

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61. During Agrobacterium infections the plant cell begins to synthesize an Arginine derivative called as

• Opines

62. Enzyme used for making cDNA

• Reverse transcriptase

63. Reagent used for visualizing DNA?

• Ethidium Bromide (EtBr)

64. Enzyme used in PCR (Polymerase Chain Reaction)

• Taq polymerase (Taq: Thermus aquaticus)

65. PCR invented by

• Kary Mullis

66. In PCR, the double stranded DNA is generally denatured by

• Temperature

67. DBM paper is used for

• Northern Blotting

68. Number of base pair units in a single turn of DNA is

• 10

69. Father of Plant Tissue culture

• Haberlandt

70. A good molecular marker must be

• Polymorphic, Heritable

71. Acid fast staining used for which bacteria?

• Mycobacterium

72. Inhibitory action of Penicillin is

• Cell wall synthesis

73. DNA staining is done by

• Feulgen staining

74. The most widely used chemical for protoplast fusion, as fusogens, is

• PEG(Poly Ethylene Glycol)

75. In order to obtained virus free plants through tissue culture the best method is

• Meristem culture

76. Endochitinase can be used as an

• Antifungal

77. Flavr Savr is a variety of

• tomato

78. The chemical used as an inducer of the vir operons is

• Acetosyringone

79.The restriction enzyme useful to the molecular biologist belongs to

• Type II

80. Which amino acids can stabilize protein structure by forming covalent cross –links between polypeptide chains ?

• Sulphur

  
  

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